The Olympic silver medallist will miss a Grand Slam tournament for the first time in two-and-a-half years.
Marketa Vondrousova has set up a gold medal clash with Belinda Bencic after thrashing Elina Svitolina.
Naomi Osaka is out of her home Olympics after a stunning straight sets defeat to Marketa Vondrousova.
Sofia Kenin is one of many players who would benefit from a deep run in Paris.
Caroline Garcia produced a brilliant display to knock out top seed Karolina Pliskova 6-1 7-6(2) at the 2020 US Open.
It is a premature end to what has been a breakthrough season for the Czech.
Caroline Wozniacki started her bid for a third title in Eastbourne with a win, while Pliskova, Ostapenko and Vondrousova also secured victories.
Playing in only her ninth grand slam main draw, the 19-year-old is through to Saturday’s final in Roland Garros.
Marketa Vondrousova will look to hold her nerve when she takes on Johanna Konta in the semi-finals at Roland Garros.
Johanna Konta advanced to her first semi-final in Rome by beating Vondrousova in three sets, while Pliskova beat Azarenka.
Simona Halep suffered a shock early loss in Rome when she was defeated in three sets by Marketa Vondrousova, while Madison Keys also lost.
Novak Djokovic unexpectedly lost to Kohlschreiber in Indian Wells, while Simona Halep was knocked out by Vondrousova.
On Sunday Czech Republic’s Marketa Vondrousova completed her dream run at the Biel Open by defeating Estonia’s Anett Kontaveit 6-4, 7-6(6), in the final. It was...